{"id":372,"date":"2021-08-08T16:13:05","date_gmt":"2021-08-08T14:13:05","guid":{"rendered":"http:\/\/directquant.eu\/?p=372"},"modified":"2021-08-08T16:13:08","modified_gmt":"2021-08-08T14:13:08","slug":"one-sample-many-applications","status":"publish","type":"post","link":"https:\/\/directquant.eu\/?p=372","title":{"rendered":"One reagent, one sample &#8211; many applications!"},"content":{"rendered":"<h2>From the same sample you can run PCR, RT-qPCR, Western Blot and measure DNA quantity.<\/h2>\n<p>The main advantage of using <a href=\"https:\/\/directquant.eu\/?page_id=340\"><strong>Dire<em>Ct<\/em>Quant 100ST<\/strong><\/a> \/ <a href=\"https:\/\/directquant.eu\/?page_id=344\"><strong>Dire<em>Ct<\/em>Quant 100W<\/strong><\/a> is that DNA, RNA and proteins can be analysed from a single sample without the need of prior separation and purification. Those reagents provide instant and complete solubilisation of the cells in the sample and permits effective extraction of:<\/p>\n<ul>\n<li>Proteins;<\/li>\n<li>Genomic DNA;<\/li>\n<li>Plasmid DNA;<\/li>\n<li>Plastid DNA;<\/li>\n<li>mitochondrial and viral DNA;<\/li>\n<li>messenger RNA;<\/li>\n<li>structural (such as ribosomal) RNA;<\/li>\n<li>transport RNA;<\/li>\n<li>microRNA;<\/li>\n<li>non-coding and viral RNA.<\/li>\n<\/ul>\n<p>The <strong>DNA <\/strong>in <a href=\"https:\/\/directquant.eu\/?page_id=340\"><strong>Dire<em>Ct<\/em>Quant 100ST<\/strong><\/a> \/ <a href=\"https:\/\/directquant.eu\/?page_id=344\"><strong>Dire<em>Ct<\/em>Quant 100W<\/strong><\/a> solubilised sample can be directly analysed by:<\/p>\n<ul>\n<li>PCR<\/li>\n<li>quantitative (Real Time) PCR (qPCR)<\/li>\n<li>quantified in solution using <a href=\"https:\/\/directquant.eu\/?page_id=348\"><strong>Dire<em>Ct<\/em>Quant DNAQ<\/strong><\/a> (Ref #DCQDNAQ)<\/li>\n<\/ul>\n<p>The <strong>RNA <\/strong>in the sample can be directly analysed by reverse transcription followed by PCR or quantitative PCR (RT-qPCR)<\/p>\n<p>The <strong>proteins <\/strong>can be directly analysed by Western blot.<\/p>\n<p><center><img loading=\"lazy\" src=\"https:\/\/directquant.eu\/wp-content\/uploads\/2017\/09\/shema2-1024x939.png\" alt=\"\" width=\"512\" height=\"470\" class=\"alignnone size-large wp-image-595\" srcset=\"https:\/\/directquant.eu\/wp-content\/uploads\/2017\/09\/shema2-1024x939.png 1024w, https:\/\/directquant.eu\/wp-content\/uploads\/2017\/09\/shema2-600x550.png 600w, https:\/\/directquant.eu\/wp-content\/uploads\/2017\/09\/shema2-300x275.png 300w, https:\/\/directquant.eu\/wp-content\/uploads\/2017\/09\/shema2-768x704.png 768w, https:\/\/directquant.eu\/wp-content\/uploads\/2017\/09\/shema2.png 1152w\" sizes=\"(max-width: 512px) 100vw, 512px\" \/> <center><\/p>\n<p><\/center><\/center><\/p>\n","protected":false},"excerpt":{"rendered":"<p>From the same sample you can run PCR, RT-qPCR, Western Blot and measure DNA quantity. The main advantage of using DireCtQuant 100ST \/ DireCtQuant 100W is that DNA, RNA and proteins can be analysed from a single sample without the need of prior separation and purification. Those reagents provide instant and complete solubilisation of the [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":595,"comment_status":"closed","ping_status":"closed","sticky":true,"template":"","format":"standard","meta":{"jetpack_publicize_message":"","jetpack_is_tweetstorm":false,"jetpack_publicize_feature_enabled":true},"categories":[12,10],"tags":[],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"https:\/\/directquant.eu\/wp-content\/uploads\/2017\/09\/shema2.png","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p91V39-60","_links":{"self":[{"href":"https:\/\/directquant.eu\/index.php?rest_route=\/wp\/v2\/posts\/372"}],"collection":[{"href":"https:\/\/directquant.eu\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/directquant.eu\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/directquant.eu\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/directquant.eu\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=372"}],"version-history":[{"count":4,"href":"https:\/\/directquant.eu\/index.php?rest_route=\/wp\/v2\/posts\/372\/revisions"}],"predecessor-version":[{"id":797,"href":"https:\/\/directquant.eu\/index.php?rest_route=\/wp\/v2\/posts\/372\/revisions\/797"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/directquant.eu\/index.php?rest_route=\/wp\/v2\/media\/595"}],"wp:attachment":[{"href":"https:\/\/directquant.eu\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=372"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/directquant.eu\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=372"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/directquant.eu\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=372"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}